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Production and characterization of monoclonal antibodies to the myelin glycolipid sulfatide
Author(s) -
Hofstetter W.,
Bologa L.,
Wetterwald A.,
Z'graggen A.,
Blaser K.,
Herschkowitz N.
Publication year - 1984
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490110402
Subject(s) - glycolipid , monoclonal antibody , myelin , chemistry , antibody , biology , immunology , biochemistry , neuroscience , central nervous system
Sulfatide is enriched in the myelin sheath and accounts for 5% of the total lipids in this membrane. In the present work we describe the production and characterization of mouse monoclonal antibodies against sulfatide. The antibodies were detected and characterized in a previously described ELISA test system. The clone AIC3IA2 produced antibodies of the IgG 3 class with high specificity for sulfatide. These antibodies showed almost no cross‐reactivity with galactocerebroside or with any of the other lipids we tested. When used with the peroxidase antiperoxidase technique the antibodies stained a cell population either in fixed or unfixed brain cell cultures, indicating a surface localization of sulfatide in the respective cell population. In double‐staining experiments the stained cell population was identified as myelin basic protein‐positive oligodendrocytes.