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A combined flat‐embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons
Author(s) -
Aldes L. D.,
Boone T. B.
Publication year - 1984
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490110104
Subject(s) - horseradish peroxidase , correlative , electron microscope , peroxidase , electron , scanning electron microscope , chemistry , materials science , optics , physics , enzyme , biochemistry , philosophy , quantum mechanics , linguistics
A simple and reproducible method is described for studying the morphology of the same neuron at the light and electron microscopic level. This method utilizes horseradish peroxidase histochemistry and a recently described flat‐embedding procedure wherein thin, aldehyde‐fixed sections are placed in resin between glass microscope slides pretreated with dimethyldichlorosilane. The significance of these combined methodologies for correlative light and electron microscopic studies of single neurons is discussed.