Premium
Rat cortex synaptic and nonsynaptic mitochondria: Enzymatic characterization and pharmacological effects of naftidrofuryl
Author(s) -
Dagani F.,
Gorini A.,
Polgatti M.,
Villa R. F.,
Benzi G.
Publication year - 1983
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490100203
Subject(s) - mitochondrion , cytochrome c oxidase , citrate synthase , glutamate receptor , malate dehydrogenase , in vivo , biochemistry , enzyme , succinate dehydrogenase , pharmacology , biology , chemistry , receptor , microbiology and biotechnology
In order to investigate the in vivo pharmacological effects of the drug naftidrofuryl, we prepared populations of synaptic and nonsynaptic mitochondria from rat brain cortex. In these different mitochondrial populations the activities of citrate synthase, malate dehydrogenase, total NADH cytochrome c reductase, cytochrome oxidase, and glutamate dehydrogenase were evaluated. Except for glutamate dehydrogenase, the specific activities of the enzymes evaluated in the „free” mitochondrial fraction were higher than those observed in the „synaptic” SM1 and SM2 mitochondrial fractions, the difference between SM1 and SM2 fractions being significant. The in vivo administration of naftidrofuryl induced few and different changes in the various mitochondrial populations.