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Acetylation of polyamines in mouse brain: Subcellular and regional distribution
Author(s) -
Ortiz J. G.,
Giacobini E.,
SchmidtGlenewinkel T.
Publication year - 1983
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490090211
Subject(s) - putrescine , cadaverine , spermidine , spermine , acetylation , biochemistry , polyamine , chemistry , olfactory bulb , biology , microbiology and biotechnology , enzyme , central nervous system , endocrinology , gene
The acetylation of putrescine, cadaverine, spermidine, and spermine was examined in different subcellular fractions and regions of the mouse brain. Acetylation activity was confined to nuclear and microsomal fractions, which can acetylate all of these compounds. These fractions catalyze the formation of N 8 but not N 1 ‐acetylspermidine. For the nuclear fraction the K m for putrescine was 3.5 mM; for cadaverine, 4.0 mM; for spermidine, 1.0 mM; and for spermine, 2.5 mM. The V max obtained were (pmol/mg protein/10 min): putrescine, 424; cadaverine, 705; spermidine, 239; and spermine, 467. The acetylation of spermidine was highest in the olfactory bulb and cerebellum. Putrescine and cadaverine acetylation were high in these areas, as well as in the midbrain. Spermine acetylation was rather uniform in all areas examined, except in the brain stem (pons‐medulla) where enzyme activity was very low.