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Modification of membrane‐bound proteins of the hippocampus and entorhinal cortex by change in behavior in rats
Author(s) -
Hydén Holger,
Lange Paul W.
Publication year - 1983
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490090106
Subject(s) - hippocampus , entorhinal cortex , hippocampal formation , membrane , chemistry , membrane protein , chloral hydrate , stimulation , cortex (anatomy) , biochemistry , biophysics , endocrinology , microbiology and biotechnology , biology , neuroscience , pharmacology
Rats were trained in an instrumental task for 2 × 25 min during 1 day and 4 days and compared with active controls with respect to membrane‐bound proteins solubilized by chloral hydrate and fractionated on polyacrylamide gels. Then 30‐μg samples of the hippocampus and entorhinal cortex were labeled by 14 C‐and 3 H‐valine. The distribution of the stained electrophoretogram was recorded by microdensitometry. The results show that the 1‐day training induced an increased synthesis of a membrane protein fraction of 50,000 mol wt already present in the brain membrane proteins of active controls. Training for 4 days resulted in an overall stimulation of the hippocampal membrane protein fractions, especially in the higher‐molecular‐weight range. The entorhinal cortex showed two stimulated membrane protein fractions, 50,000 and 120,000 mol wt. Together with previous studies, this study makes it probable that training to establish a new behavior induces a modulation of both soluble and membrane‐bound protein patterns in the hippocampus and the entorhinal cortex with a time phase retardation for the latter.