Premium
[ 3 H]spiperone binding sites in rat primary glial cultures, C6 glioma, and B104 neuroblastoma
Author(s) -
Severson J. A.,
de Vellis J. S.,
Finch C. E.
Publication year - 1983
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490090104
Subject(s) - spiperone , striatum , binding site , biology , chemistry , microbiology and biotechnology , neuroscience , dopamine , biochemistry , dopamine receptor
Binding sites for [ 3 H]spiperone were detected on membranes from primary glial cultures from neonatal rat cortex and striatum and from the C6 glioma cells. [ 3 H]Spiperone binding was displacable by d‐butaclamol. However, competition studies suggest that [ 3 H]spiperone binding to primary glial cultures was mainly to serotonergic sites, and binding to the C6 glioma was alpha‐adrenergic. No specific binding was detected to membranes from B104 neuroblastoma cells. Although [ 3 H]spiperone binding to glial sites on whole striatum under generally used conditions is small (about 10%), the striatal glial hyperactivity that is often associated with neuronal degeneration could lead to an overestimation of presumed neuronal binding sites for dopaminergic ligands.