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Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system
Author(s) -
Simpson D. L.,
Morrison R.,
de Vellis J.,
Herschman H. R.
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490080233
Subject(s) - epidermal growth factor , thymidine , astrocyte , biology , neuroglia , growth factor , cell culture , chemically defined medium , central nervous system , microbiology and biotechnology , cell growth , biochemistry , endocrinology , in vitro , receptor , genetics
Binding of 125 I‐epidermal growth factor (EGF) to purified populations of rat astrocytes, oligodendrocytes, and neurons was measured. Astrocytes bound 40,000–100,000 EGF molecules per cell, while oligodendrocytes bound only 6,000–10,000 EGF molecules per cell. In contrast, neurons had little or no capacity to bind 125 I‐EGF. EGF alone was able to stimulate incorporation of tritiated thymidine fivefold in purified astrocyte cultures incubated in serum‐free medium. When EGF was added to the previously described chemically defined medium for astrocytes, incorporation of tritiated thymidine in purified astrocytes was equivalent to that observed in medium containing 10% fetal calf serum. Addition of EGF to this chemically defined medium also doubled the proliferative capability of the medium for cultured astrocytes. EGF was maximally effective in stimulating 3 H‐thymidine incorporation at concentrations between 1–10 ng/ml.