Premium
Characteristics of the association of nerve growth factor with primary cultures of rat sympathetic neurons
Author(s) -
Hawrot Edward
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490080211
Subject(s) - nerve growth factor , neuroscience , primary (astronomy) , association (psychology) , primary culture , sympathetic nervous system , medicine , endocrinology , biology , psychology , cell culture , genetics , physics , receptor , blood pressure , astronomy , psychotherapist
Long‐term primary cultures of rat sympathetic neurons require NGF for survival and development. The kinetics of the interaction of 125 I‐NGF with sympathetic neuron cultures suggests the presence of diffusional barriers preventing a determination of true dissociation and association rate constants. Although the observed rate constants do not accurately reflect the microscopic interaction of NGF with receptor, the ratio of the observed rate constants does provide a good estimate of the K D . This value (1 × 10 −9 M) agrees with earlier steady state measurements of the K D . The association of 125 I‐NGF with neuronal cultures is temperature‐dependent with internalization and retrograde transport occurring at 37°C. The retrograde transport of 125 I‐NGF in compartmentalized neuronal cultures is concentration dependent and saturates at about 100 ng/ml (4 × 10 −9 M). The amount of 125 I‐NGF accumulated by retrograde transport appears to be increased subsequent to a period of NGF‐starvation. The increase in uptake does not appear to be due to an increase in NGF receptor number since the number of binding sites is not greatly increased upon NGF starvation.