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Acceleration of the biosynthesis of rat striatal dopamine by incubation and by administration of γ‐butyrolactone
Author(s) -
Dyck Lillian E.,
Kazakoff Clement W.
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490080109
Subject(s) - dopamine , incubation , apomorphine , chemistry , endocrinology , tyrosine hydroxylase , medicine , pharmacology , tyrosine , amphetamine , biology , biochemistry , dopaminergic
The concentration of dopamine in striatal slices obtained from rats treated with an MAO inhibitor was increasd significantly by a 50‐minute incubatio in oxygenated Krebs buffer at 37°C. Administration of α‐methyl‐p‐tyrosine did not antagonize this small increase in dopamine. The extent of the incubatio‐induced increase was much greater in tissue obtained from control rats and was also greater than that resulting from the intraperitoneal administration of γ‐butyrolactone (GBL). Prior administration of α‐methyl‐p‐tyrosine, d‐amphetamine, apomorphine, or lergotrile significantly antagonized the increases in striatal dopamine levels induced by GBL administration or by incubation of striatal slices obtained from control rats. The prior administration of phenylethylamine, however, antagonized the incubation‐induced, but not the GBL‐induced, increase in striatal dopamine. In conclusion, the effect of incubating striatal slices on their dopamine levels was similar to that of administering GBL in that the incubation enhanced the activity of tyrosine hydroxylase that in turn produced an accelerated synthesis of dopamine.