Premium
Tay‐Sachs disease and normal cerebellar cells in culture: Elevated levels of lysosomal enzymes in Tay‐Sachs disease cells
Author(s) -
Hoffman Linda M.,
Brooks Steven E.,
Schneck Larry
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490080108
Subject(s) - tay sachs disease , acid phosphatase , enzyme , lysosomal storage disease , hexosaminidase , lysosome , biology , mannose 6 phosphate receptor , microbiology and biotechnology , immunology , endocrinology , disease , chemistry , medicine , biochemistry
Abstract Tay‐Sachs disease and normal fetal cerebellar cells were maintained in culture for up to four weeks. Elevated levels of the lysosomal enzymes hexosaminidase B, β‐galactosidase, and acid phosphatase were observed in the Tay‐Sachs cells in long term cultures although elevated lysosomal enzyme levels were not observed in the normal cultures. Similarly, elevated lysosomal enzyme activities were observed in Tay‐Sachs cultures which had been treated with Concanavalin A.