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Toxic effects of ouabain and food and cosmetic dyes on nerve growth factor‐promoted differentiation of neurites in culture
Author(s) -
Morris Stephen J.,
Chronwall Bibie M.
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490070311
Subject(s) - neurite , nerve growth factor , ouabain , chemistry , in vitro , xanthene , fluorescein , neurotoxicity , microbiology and biotechnology , pharmacology , biochemistry , biology , toxicity , photochemistry , fluorescence , receptor , physics , organic chemistry , quantum mechanics , sodium
The toxic effects of ouabain and various fluorescein analogs have been tested on nerve growth factor‐promoted (NGF‐promoted) outgrowth of neurites from embryonic chick sensory ganglia embedded in a matrix of native collagen. Ouabain inhibits outgrowth with an IC 50 of approximately 5 μM. Erythrosin B (USFD&C Red 3) and other fluorescein derivative dyes brominated or iodinated on the xanthene ring also inhibit neurite outgrowth with IC 50 'S as low as 10 μm and promote early retraction of growing neurites. The effects of the dyes are due, at least in part, to photooxidation of the NGF, but they may also act directly on the cells. The culture system should prove useful as both a general and a specific in vitro assay for neurotoxicity.