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Effects of methadone on ornithine decarboxylase and cyclic nucleotide phosphohydrolase in neuronal and glial cell cultures
Author(s) -
Vernadakis Antonia,
Estin Charles,
Gibson D. Ann,
Amott Susan
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490070203
Subject(s) - cyclic nucleotide , ornithine decarboxylase , nucleotide , chemistry , methadone , biochemistry , biology , enzyme , microbiology and biotechnology , pharmacology , gene
Abstract Mixed neuronal and nonneuronal cell cultures were obtained from 8‐day‐old chick embryos cerebral hemispheres and glial‐enriched cultures were obtained from fifteen‐day‐old chick embryo cerebral hemispheres. Cultures were exposed to methadone, a narcotic drug, from days four to six. The activity of ornithine decarboxylase (ODC) was determined at day eight and the activity of cyclic nucleotide phosphohydrolase (CNP) was determined at day fifteen. Both ODC and CNP activity were higher in mixed neuronal‐nonneuronal cell cultures treated with methadone as compared to control. No effect was observed in the neuronal‐enriched or glial‐enriched cultures. These findings are interpreted to reflect that neuronal‐glial interaction is important in the response of primary neural cells to methadone.