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Electroblot analysis of the myelin proteolipid protein
Author(s) -
Macklin Wendy B.,
Braun Peter E.,
Lees Marjorie B.
Publication year - 1982
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490070102
Subject(s) - myelin , proteolipid protein 1 , myelin proteolipid protein , nitrocellulose , polyacrylamide gel electrophoresis , chemistry , myelin basic protein , biochemistry , biology , central nervous system , enzyme , neuroscience , membrane
Abstract The myelin proteolipid has been studied by the electroblot method of Towbin et al [1979]. Samples were separated by SDS–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and incubated with proteolipid antibody. The proteolipid band could be identified immunologically in CNS myelin and in whole brain homogenates. No proteolipid was detected in PNS myelin. Proteolipid from human, bovine, rat, and mouse myelin all cross‐react when analyzed by this method. No cross‐reactivity was indicated between proteolipid and myelin basic protein.