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Studies in neuronal ceroid‐lipofuscinosis: Heterogeneous nature of neuronal autofluorescent lipopigments
Author(s) -
Garg Hari S.,
Awasthi Yogesh C.,
Srivastava Satish K.
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060611
Subject(s) - fluorescence , neuronal ceroid lipofuscinosis , chemistry , brain tissue , lipofuscin , thin layer chromatography , chromatography , biology , biochemistry , anatomy , physics , quantum mechanics , gene
A method has been developed for the complete extraction and fractionation of the autofluorescent lipopigments present in the neuronal tissue of Batten‐Spielmeyer‐Vogt (BSV) patient and normal subject, respectively. The fluorescent lipopigments that were about three‐fold higher in BSV patient's brain as compared with normal subjects were separated into at least 7 fluorometrically and chromatographically distinct fractions by thin layer chromatography (TLC). Although the fluorescent lipopigments of the brain tissue of BSV patient and normal subject showed identical pattern of separation on TLC, the fluorescence spectra of the individual lipopigment fractions from BSV patient was different from those of the corresponding fractions from the normal subject. The ultraviolet spectral studies with individual fluorescent lipopigment fractions indicated the presence of highly unsaturated conjugated chromophore.