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The vimentin‐GFA protein transition in rat neuroglia cytoskeleton occurs at the time of myelination
Author(s) -
Dahl Doris
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060608
Subject(s) - vimentin , cytoskeleton , glial fibrillary acidic protein , intermediate filament , neuroglia , microbiology and biotechnology , immunocytochemistry , gfap stain , biology , chemistry , neuroscience , biochemistry , cell , immunohistochemistry , immunology , central nervous system , endocrinology
Extraction with Triton, a nonionic detergent, is a common procedure to prepare intermediate filament enriched fractions from cells maintained in culture. The Triton‐Insoluble fraction is called a cytoskeletal preparation. Using this procedure, we previously demonstrated that vimentin and glial fibrillary acidic protein (GFA) are major cytoskeletal proteins of neuroglia, in newborn and adult rat brain, respectively. In the present communication we report that the vimentin‐GFA transition in rat brain occurs during the 2nd‐3rd week, ie, at the time of rapid myelination when dividing glioblasts in white matter differentiate into oligodendroglia and interfascicular astrocytes.