Premium
Endogenous activation of guanylate cyclase in synaptosomal soluble fraction from rat brain
Author(s) -
Yoshikawa Kazuaki,
Kuriyama Kinya
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060607
Subject(s) - gucy1a3 , gucy1b3 , guanylate cyclase , chemistry , biochemistry , endogeny , guanosine , fraction (chemistry) , guanylate cyclase 2c , cyclase , enzyme , cyclic guanosine monophosphate , chromatography , organic chemistry , nitric oxide
Guanylate cyclase in crude mitochrondrial (P 2 ) soluble fraction prepared from rat brain, obtained by a hypo‐osmotic treatment of P 2 , showed extremely higher activity than that in the same fraction from other organs. In addition the soluble fraction obtained from synaptosomes (P 2 ‐B) contained the highest enzyme activity among other subfractions of the cerebral P 2 examined. Guanylate cylase activity in the synaptosomal soluble fraction was, however, markedly suppressed by various compounds reacting with free radicals. These results suggest that guanylate cyclase in the synaptosomal soluble fraction may be activated endogenously by a free radical and involved in the regulatory mechanisms for cyclic guanosine monophosphate (cyclic GMP) at presynaptic terminals.