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Detection of antibodies to central nervous system antigens by solid phase radioimmunoassay
Author(s) -
Linthicum D. Scott,
Mackay Ian R.,
Wilson Anne,
Horvath Leonie B.,
Carnegie Patrick R.
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060503
Subject(s) - radioimmunoassay , antigen , antibody , antiserum , myelin basic protein , central nervous system , myelin , cerebrospinal fluid , glial fibrillary acidic protein , biology , galactocerebroside , immunology , microbiology and biotechnology , chemistry , oligodendrocyte , immunohistochemistry , biochemistry , endocrinology , neuroscience
A solid phase radioimmunoassay is described which employs 125 I‐protein‐A to detect the presence of antibodies against a panel of cellular and soluble central nervous system (CNS) specific antigens coated onto polyvinylchloride Microtiter plates. Serum antibodies from rabbits immunized against myelin, myelin basic protein (MBP), glial acidic fibrillary protein (GFAP), astroglioma cells, and cerebellar cells were easily detected, and high specificity for each antiserum and antigen was also demonstrable. The assay is applicable to cerebrospinal fluid (CSF) from patients with neurological diseases to detect antibodies against CNS‐specific antigens. The assay should be useful for examining cell lines derived from CNS tissue for the presence of brain proteins.