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Taurine binding to membranes from rat brain regions
Author(s) -
LöpezColomé A. M.,
PasantesMorales H.
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060405
Subject(s) - taurine , olfactory bulb , bicuculline , membrane , strychnine , biophysics , binding site , postsynaptic potential , cerebellum , chemistry , dorsal root ganglion , cerebral cortex , biochemistry , glycine , biology , neuroscience , central nervous system , receptor , gabaa receptor , amino acid , spinal cord
[ 3 H]‐taurine binding to membranes from different regions from rat brain was studied. Binding to membranes from cerebral cortex and its subcellular fractions, hypothalamus, olfactory bulb and cerebellum was measured. Binding to membranes from dorsal root ganglion was also determined. Na + ‐dependent taurine binding was consistently observed in all the membranes except those from dorsal root ganglion. A K D = 4.06 μM was obtained for binding to membranes from cerebral cortex. Na + ‐dependent taurine binding was displaced by 20 μM strychnine or bicuculline. Na + ‐independent taurine binding with properties corresponding to a postsynaptic interaction could not be detected in any of the regions studied. The possibility of Na + ‐dependent taurine binding, representing binding to uptake sites or to postsynaptic receptors for GABA and glycine, is discussed.