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Monoclonal antibodies against mouse nerve growth factor produced by somatic cell hybrids
Author(s) -
Businaro Rita,
Butler Richard H.,
Rubenstein Allan E.,
Revoltella Roberto P.
Publication year - 1981
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490060109
Subject(s) - nerve growth factor , antibody , monoclonal antibody , antigen , biology , microbiology and biotechnology , radioimmunoassay , complement fixation test , spleen , immunology , endocrinology , serology , biochemistry , receptor
Spleen leukocytes from rats and mice immunized against mouse 2.5S nerve growth factor (NGF) and peripheral blood leukocytes from rabbits hyperimmunized against the same antigen were fused with the mouse plasmacytoma P3 × 63Ag8. Hybridomas were screened by immunological assays (microcomplement fixation test and solid phase radioimmunoassay) for production of antibodies that reacted with NGF. Significant variations were seen between culture fluids from different hybrid cells. In addition, most but not all hybridoma antibodies that reacted immunologically with NGF prevented neurite outgrowth from 8‐day chick embryo sensory ganglia explants after binding to NGF. These results suggest that the hybridoma antibodies produced by the different clones react with different antigenic sites on the NGF molecule.