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Cysteinesulfinate decarboxylase activity as an index of taurine‐containing structures
Author(s) -
Staines Wm. A.,
Benjamin A. M.,
McGeer E. G.
Publication year - 1980
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490050610
Subject(s) - glutamate decarboxylase , taurine , in vivo , carboxy lyases , in vitro , lesion , biochemistry , chemistry , distribution (mathematics) , endogeny , enzyme , biology , amino acid , pathology , medicine , mathematical analysis , microbiology and biotechnology , mathematics
The distribution of cysteinesulfinic acid decarboxylase (CSAD) activity has been studied for some time on the assumption that this activity was a marker for taurine‐containing structures in the CNS. We have found that various in vivo and in vitro treatments of CNS tissues result in parallel changes in the activities of CSAD and glutamic acid decarboxylase (GAD). This suggests that the assay for CSAD is in fact measuring predominantly GAD activity. This hypothesis is tested by lesion, regional distribution, kinetic, inhibitor, and stability studies.