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Simultaneous fluorescent retrograde axonal tracing and immunofluorescent characterization of neurons
Author(s) -
Van Der Kooy Derek,
Steinbusch Harry W. M.
Publication year - 1980
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490050603
Subject(s) - fluorescence , propidium iodide , immunofluorescence , immunocytochemistry , retrograde tracing , biology , fluorescein , chemistry , anatomy , microbiology and biotechnology , dorsum , antibody , biochemistry , immunology , optics , apoptosis , physics , programmed cell death , endocrinology
A simple method is described for simultaneously double labeling single neurons with fluorescent retrograde axonal tracers and immunofluorescence using an antibody to serotonin. Caudate‐putamen injection of orange fluorescent propidium iodide, in particular, proved effective in retrogradely labeling dorsal raphe cell bodies. These same perikarya could be stained green with the indirect immunofluorescent method, using green fluorescent fluorescein isothiocynate (FITC) as the second antibody.