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Role of glycoproteins in the uptake of L‐ 14 C ‐ leucine by a synaptosomal fraction
Author(s) -
Rossowska Magdalena
Publication year - 1980
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490050402
Subject(s) - concanavalin a , neuraminidase , leucine , glycoprotein , biochemistry , chemistry , membrane , amino acid , enzyme , in vitro
The present study deals with the effect of modification of membrane glycoproteins on the accumulation of amino acids by isolated synaptosomal fractions, measured by the uptake of 14 C‐leucine. Superficial sugar receptors were modified by concanavalin A, neuraminidase, and neuraminidase followed by concanavalin A binding. It is demonstrated that neuraminidase treatment followed by concanavalin A binding results in significant diminution of 14 C‐leucine uptake. A similar effect was observed in experimental hypoxia followed by concanavalin A binding to isolated synaptosomal fractions. In this case, however, (Na + K + ) –ATPase activity remained unchanged. These results seem to indicate that 14 C−leucine uptake was dependent on integrity of glycoprotein structure of synaptosomal membranes.