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Mass spectrometric identification of Cu, Zn, Fe, Co, Mn, Mg, and Pb in mammalian brain
Author(s) -
Hui K.S.,
Davis B. A.,
Boulton A. A.
Publication year - 1979
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490040303
Subject(s) - chemistry , striatum , white matter , caudate nucleus , thalamus , cerebral cortex , cerebellum , human brain , hypothalamus , putamen , cortex (anatomy) , biophysics , biology , endocrinology , neuroscience , medicine , magnetic resonance imaging , dopamine , radiology
Combining the techniques of thin‐layer chromatography (TLC) and mass spectrometry, we unambiguously identified the trace metals Cu, Zn, Fe, Pb, Mn, Co, and Mg in the brain of a female human who had no evidence of any pathologic disease in the central neveous system, and in brains from mouse, rat, guinea pig, and rabbit. These trace metals were also found in anatomic regions of human brain: cortex (gray), cortex (white), caudate nucleus, putamen, hippocampus, and thalamus, and in anatomic regions of rat brain: hypothalamus, cerebellum, stem striatum, and “the rest.” The metals were characterized from the color and R f values of their tetraphenylporphyrin chelates on TLC and from the mass and pattern of molecule ion cluster of the mass spectrum. The unexpected presence of lead in the brain is discussed.