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The effect of morphine tolerance on the incorporation of 3 H‐leucine into proteins of rat synaptic membranes
Author(s) -
Clouet Doris H.,
Ratner Milton
Publication year - 1979
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490040203
Subject(s) - synaptic vesicle , membrane , vesicle , membrane protein , leucine , chemistry , biochemistry , postsynaptic potential , postsynaptic density , polyacrylamide gel electrophoresis , biophysics , microbiology and biotechnology , biology , amino acid , enzyme , receptor
The rate of incorporation of 3 H‐leucine into proteins of the synaptic junctional and nonjunctional membranes and synaptic vesicles of rat brain has been examined in control and morphine‐tolerant rats. There are no discernible differences between control and tolerant animals in amount of protein as measured by densitometric tracings of dye‐stained proteins separated by acrylamide gel electrophoresis from the three membrane fractions of whole brain areas. However, there are differences in the turnover of membrane protein: three vesicle protein bands and one junctional‐membrane protein band are significantly more highly labeled, and one junctional‐membrane protein is significantly less highly labeled by 3 H‐leucine in samples from tolerant rats. Of these, the two junctional‐membrane proteins can be tentatively identified as components of the postsynaptic densities, and one of the vesicle proteins as tubulin.