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A regional study of electrophoretically separated proteins from the crude synaptosomal fractions of the developing rat brain
Author(s) -
Davis Leonard G.,
Brunngraber Eric G.,
Routtenberg Aryeh
Publication year - 1979
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490040105
Subject(s) - polyacrylamide gel electrophoresis , differential centrifugation , hippocampal formation , cerebellum , centrifugation , sucrose gradient , biology , chemistry , biochemistry , endocrinology , enzyme
Albino rats of 1, 5, 9, 16, and 25 days of age were sacrificed, and six brain regions were dissected free. Crude synaptosomal preparations from each brain region and age were obtained by differential centrifugation. Protein content in these fractions from the six brain regions increased almost threefold between 1 and 25 days of age. The preparations were delipidated, solubilized, and subjected to polyacrylamide gel electrophoresis. The densitometric patterns of five samples from each age and brain region were analyzed for changes in the relative amount of stained protein bands. Of the polyacrylamide gel‐separated protein bands, 16 regional differences at specific ages and 20 developmental changes in specific brain regions were found to be statistically significant. The hippocampal region showed the greatest number of statistical changes in the protein pattern as a function of age, whereas the cerebellum showed none. The results suggest the importance of considering regional differences in studies of biochemical developmental changes.