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Stereospecific opiate binding in human erythrocyte membranes and changes in heroin addicts
Author(s) -
Abood Leo G.,
Atkinson Holly G.,
Macneil Mary
Publication year - 1976
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490020511
Subject(s) - opiate , (+) naloxone , stereospecificity , morphine , membrane , chemistry , pharmacology , potency , heroin , trypsin , medicine , in vitro , biochemistry , opioid , drug , enzyme , receptor , catalysis
Stereospecific opiate binding has been demonstrated in human erythrocyte membranes, having a K d of 9 · 10 –9 M. In most respects the binding characteristics resemble those of snyaptic membranes. These included the correlation of binding affinity and pharmacological potency of opiates; competition by naloxone; inhibition by Ca 2+ and Na + ; and sensitivity to phospholipases and tryspin. A comparison of stereospecific opiate binding in control human subjects and heroin addicts revealed a 43% increase in the addict group.