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Effect of serum deprivation on myelinating mouse cerebellum cultures
Author(s) -
Kim Seung U.
Publication year - 1976
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490020408
Subject(s) - cerebellum , myelin , radioimmunoassay , in vitro , medicine , endocrinology , cholinesterase , biology , myelin basic protein , chemistry , biochemistry , central nervous system
In order to investigate the effect of nutrient deprivation on brain development, mouse cerebellum cultures were grown in nutrient media with reduced serum concentration. After 9–18 days in vitro, experimental cultures grown in media with low serum concentration exhibited a delayed and retarded myelination. Electron microscopic examination of experimental cultures revealed profiles of deficient myelination, but showed normal neuronal and synaptic structures. The amount of myelin basic protein measured by radioimmunoassay was markedly reduced in experimental cultures. Activity of cholinesterase in these experimental cultures was also decreased.