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Cellular and subcellular distribution of the S‐100 protein in rabbit and rat central nervous system
Author(s) -
Haglid K. G.,
Hamberger A.,
Hansson H.A.,
Hydén H.,
Persson L.,
Rönnbäck L.
Publication year - 1976
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490020302
Subject(s) - immunoelectron microscopy , central nervous system , nervous system , microbiology and biotechnology , subcellular localization , biology , ultrastructure , postsynaptic potential , membrane , mitochondrion , chemistry , biochemistry , immunohistochemistry , biophysics , cytoplasm , neuroscience , anatomy , immunology , receptor
The cellular and subcellular distribution of the S‐100 protein in rabbit and rat central nervous system was studied both quantitatively and qualitatively. Microcomplement fixation estimations on bulk‐prepared neuronal and glial cells showed at least five to six times higher amounts of water‐soluble S‐100 protein. High levels of S‐100 protein were found in mitochondria and soluble protein fractions. Immunoelectron microscope investigations demonstrated S‐100 protein in neuronal structures, such as the postsynaptic membrane and part of the plasma membrane. Among glial cells the astrocytic filaments contained high levels of S‐100 protein. S‐100 was also found in most subcellular membranes of astrocytes and oligodendrocytes.