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The chronic and acute effects of ethanol on adenosine triphosphatase activity in cultured astroblast and neuroblastoma cells
Author(s) -
Syapin P. J.,
Stefanovic V.,
Mandel P.,
Noble E. P.
Publication year - 1976
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490020205
Subject(s) - ethanol , atpase , hamster , cell culture , neuroblastoma , neuroblast , biochemistry , biology , cell , enzyme , chemistry , microbiology and biotechnology , genetics , neurogenesis
Mg 2+ ATPase and (Na + K + )ATPase activities were measured in clonal line NN hamster astroblasts and in clonal lines M 1 and N 1E‐115 mouse neuroblastoma cells after the cells had been subjected to the acute and chronic actions of 100 mM ethanol. Exposure of the astroblasts to ethanol for periods as long as 68 days produced an increase in total cellular Mg 2+ ATPase activity, as measured in cell homogenates; however, activity reverted to control levels upon withdrawal of ethanol. Chronic exposure of clonal line N 1E‐115 neuroblastoma cells to ethanol produced an increase in Mg 2+ ATPase and (Na + K + )ATPase activities. In contrast, the activities of both ATPases of clonal line M1 neuroblasts were unaffected by chronic exposure to ethanol. Acute exposure of cell homogenates to 100 mM ethanol inhibited Mg 2+ ATPase and (Na + K + )ATPase of astroblasts but not that of neuroblastoma cells. These findings suggest that neural cells in culture may serve as useful models for studying the effects of ethanol on specific cell types.