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The use of microchemical techniques for the identification of new transmitter molecules in neurons
Author(s) -
Giacobini E.
Publication year - 1975
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490010102
Subject(s) - neurotransmitter , identification (biology) , neurotransmitter agents , neuroscience , computational biology , neurotransmitter systems , transmitter , biology , chemistry , computer science , central nervous system , dopamine , computer network , channel (broadcasting) , botany
The author reviews available microtechniques and results on cellular analysis of single neurons emphasizing the identification of putative neurotransmitter molecules. Highly sensitive microquantitative methods for identification and assay of known and putative neurotransmitters have been developed within the last 5 years. Several of the methods reported in the article fulfill the requirements of both specificity and sensitivity for exploring and measuring the level of transmitter molecules in single neurons. Some of the available techniques, mostly those which associate GC or TLC with MS, have been able to unambiguously demonstrate the presence of putative neurotransmitter molecules, or at least of substances which are strongly suspected of fulfilling a functional role in the nervous system.