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Intrinsic circadian timekeeping properties of the thalamic lateral geniculate nucleus
Author(s) -
Chrobok Lukasz,
Pradel Kamil,
Janik Marcelina Elzbieta,
Sanetra Anna Magdalena,
Bubka Monika,
Myung Jihwan,
Ridla Rahim Amalia,
Klich Jasmin Daniela,
JeczmienLazur Jagoda Stanislawa,
PalusChramiec Katarzyna,
Lewandowski Marian Henryk
Publication year - 2021
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.24973
Subject(s) - suprachiasmatic nucleus , neuroscience , lateral geniculate nucleus , optogenetics , circadian clock , circadian rhythm , biology , geniculate , clock , diencephalon , nucleus , hypothalamus , retina
Circadian rhythmicity in mammals is sustained by the central brain clock—the suprachiasmatic nucleus of the hypothalamus (SCN), entrained to the ambient light–dark conditions through a dense retinal input. However, recent discoveries of autonomous clock gene expression cast doubt on the supremacy of the SCN and suggest circadian timekeeping mechanisms devolve to local brain clocks. Here, we use a combination of molecular, electrophysiological, and optogenetic tools to evaluate intrinsic clock properties of the main retinorecipient thalamic center—the lateral geniculate nucleus (LGN) in male rats and mice. We identify the dorsolateral geniculate nucleus as a slave oscillator, which exhibits core clock gene expression exclusively in vivo . Additionally, we provide compelling evidence for intrinsic clock gene expression accompanied by circadian variation in neuronal activity in the intergeniculate leaflet and ventrolateral geniculate nucleus (VLG). Finally, our optogenetic experiments propose the VLG as a light‐entrainable oscillator, whose phase may be advanced by retinal input at the beginning of the projected night. Altogether, this study for the first time demonstrates autonomous timekeeping mechanisms shaping circadian physiology of the LGN.