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Author(s) -
Jun Liu
Publication year - 2015
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23680
Subject(s) - luciferase , green fluorescent protein , in vitro , microbiology and biotechnology , in vivo , fusion protein , chemistry , biology , cell culture , biochemistry , recombinant dna , gene , transfection , genetics
NS/PCs obtained from the striata of fetuses of transgenic mice with ubiquitous expression of ff Luc‐cp156, a fusion protein of firefly luciferase and Venus protein, are used in this study Hara‐Miyauchi et al., 2012). The obtained cells can be easily detected both in vitro and in vivo by fluorescence of Venus (a variant of GFP), and also by luminescence from luciferase. This image was taken in an in vitro differentiation assay of derived NS/PCs, and shows that all the cells are positive for GFP (green), while only a portion are positive for GFAP (red), confirming the multipotency of the obtained NS/PCs.