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Role of oxidative stress in surgical cavernous nerve injury in a rat model
Author(s) -
Wang Hui,
Ding XieGang,
Li ShiWen,
Zheng Hang,
Zheng XinMin,
Navin Shrestha,
Li Lu,
Wang XingHuan
Publication year - 2015
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23545
Subject(s) - oxidative stress , glutathione peroxidase , malondialdehyde , endocrinology , nerve injury , medicine , nitrotyrosine , crush injury , nissl body , superoxide dismutase , chemistry , anesthesia , staining , pathology , nitric oxide synthase , surgery , nitric oxide
This study investigates the role of oxidative stress in surgical cavernous nerve (CN) injury in a rat model. Eighty‐four male Sprague‐Dawley rats were randomly divided into three groups: group 1, sham‐operated rats; group 2, bilateral CN‐crushed rats; and group 3, bilateral CN‐transection‐and‐sutured‐immediately rats. Oxidative stress was evaluated by malondialdehyde levels, super oxide dismutase (SOD) activities, and glutathione peroxidase (GPX) activities in serum. Erectile function was assessed by CN electrostimulation at 3 months with mean maximal intracavernous pressure (ICP) and maximal ICP per mean arterial pressure. Nerve injury was assessed by toluidine blue staining of CNs and nicotinamide adenine dinucleotide phosphate (NADPH)‐diaphorase staining of penile tissue. GPX protein expression and nitrotyrosine‐3 (NT‐3) levels in penile tissue were measured. Erectile function and the number of myelinated axons of CNs and NADPH‐diaphorase‐positive nerve fibers were statistically decreased between groups, from sham to crush to transection. For markers, both nerve‐injury groups showed increased oxidative stress markers at early time points, with the transection group showing greater oxidative stress than the crushed group and values normalizing to sham levels by week 12. GPX expression and NT‐3 levels in penile tissue were in concordance with the results of SOD and GPX. These results show that oxidative stress plays an important role in injured CNs, and different methods of CN injury can lead to different degrees of oxidative stress in a rat model. © 2015 Wiley Periodicals, Inc.