z-logo
Premium
Inhibition of anandamide hydrolysis enhances noradrenergic and GABAergic transmission in the prefrontal cortex and basolateral amygdala of rats subjected to acute swim stress
Author(s) -
Bedse Gaurav,
Romano Adele,
Tempesta Bianca,
Lavecchia Michele A.,
Pace Lorenzo,
Bellomo Antonello,
Duranti Andrea,
Micioni Di Bonaventura Maria Vittoria,
Cifani Carlo,
Cassano Tommaso,
Gaetani Silvana
Publication year - 2015
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23539
Subject(s) - basolateral amygdala , anandamide , microdialysis , chemistry , endocannabinoid system , fatty acid amide hydrolase , cannabinoid receptor , endocrinology , medicine , prefrontal cortex , cannabinoid , neurotransmitter , pharmacology , extracellular , receptor , neuroscience , biology , antagonist , biochemistry , cognition
Limbic forebrain endocannabinoid (eCB) signaling is critically involved in stress integration by modulating neurotransmitters release. The purpose of this study was to examine, by brain microdialysis, the effects of fatty acid amide hydrolase (FAAH) inhibition on noradrenergic and γ‐aminobutyric acid (GABA)‐ergic neurotransmission in the prefrontal cortex (PFC) and basolateral amygdala (BLA) of rats subjected to a 20‐min swim stress. Microdialysis started on stress‐ and drug‐naïve rats that were treated with the FAAH inhibitor URB597 (0.1 or 0.3 mg/kg) 30 min before undergoing the stress procedure. Dialysate samples were collected every 20 min from the beginning of the experiment. Concentrations of noradrenaline (NA) and GABA were determined by HPLC coupled to electrochemical and fluorescence detection, respectively. We found that neither URB597 treatment nor 20 min of swim stress exposure per se altered NA and GABA extracellular levels in PFC or BLA. Interestingly, rats treated with 0.1 mg/kg of URB597 followed by 20 min of stress showed significantly higher NA and GABA levels in PFC and BLA. These effects were absent in rats treated with 0.3 mg/kg URB597, indicating a dose‐specific effect. Moreover, we found that the pretreatment with the CB1 receptor antagonist rimonabant blocked the URB597 effects on NA and GABA release in PFC and BLA of animals subjected to forced swimming. The present study might provide an important first step toward understanding the mechanisms through which URB597 modulates stress‐induced neuroendocrine secretion and behavioral coping strategies. © 2015 Wiley Periodicals, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here