Premium
Roles of astrocytic Na + ,K + ‐ATPase and glycogenolysis for K + homeostasis in mammalian brain
Author(s) -
Hertz Leif,
Gerkau Niklas J.,
Xu Junnan,
Durry Simone,
Song Dan,
Rose Christine R.,
Peng Liang
Publication year - 2015
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23499
Subject(s) - glycogenolysis , endocrinology , medicine , glutamate receptor , astrocyte , extracellular , stimulation , chemistry , biology , biophysics , biochemistry , receptor , metabolism , central nervous system
Neuronal excitation increases extracellular K + concentration ([K + ] o ) in vivo and in incubated brain tissue by stimulation of postsynaptic glutamatergic receptors and by channel‐mediated K + release during action potentials. Convincing evidence exists that subsequent cellular K + reuptake occurs by active transport, normally mediated by Na + ,K + ‐ATPase. This enzyme is expressed both in neurons and in astrocytes but is stimulated by elevated [K + ] o only in astrocytes. This might lead to an initial K + uptake in astrocytes, followed by Kir4.1‐mediated release and neuronal reuptake. In cell culture experiments, K + ‐stimulated glycogenolysis is essential for operation of the astrocytic Na + ,K + ‐ATPase resulting from the requirement for glycogenolysis in a pathway leading to uptake of Na + for costimulation of its intracellular sodium‐binding site. The astrocytic but not the neuronal Na + ,K + ‐ATPase is additionally stimulated by isoproterenol, a β‐adrenergic agonist, but only at nonelevated [K + ] o . This effect is also glycogenolysis dependent and might play a role during poststimulatory undershoots. Attempts to replicate dependence on glycogenolysis for K + reuptake in glutamate‐stimulated brain slices showed similar [K + ] o recovery half‐lives in the absence and presence of the glycogenolysis inhibitor 1,4‐dideoxy‐1,4‐imino‐d‐arabinitol. The undershoot was decreased, but to the same extent as an unexpected reduction of peak [K + ] o increase. A potential explanation for this difference from the cell culture experiments is that astrocytic glutamate uptake might supply the cells with sufficient Na + . Inhibition of action potential generation by tetrodotoxin caused only a marginal, nonsignificant decrease in stimulated [K + ] o in brain slices, hindering the evaluation if K + reaccumulation after action potential propagation requires glycogenolysis in this preparation. © 2014 W iley Periodicals, Inc .