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Palmitic acid affects proliferation and differentiation of neural stem cells in vitro
Author(s) -
Wang Zhen,
Liu Dexiang,
Zhang Qun,
Wang Jianmei,
Zhan Jingmin,
Xian Xiuying,
Du Zhaoxia,
Wang Xueer,
Hao Aijun
Publication year - 2014
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23342
Subject(s) - neural stem cell , microbiology and biotechnology , in vitro , bromodeoxyuridine , reactive oxygen species , cell growth , biology , transcription factor , chemistry , stem cell , biochemistry , gene
High‐lipid diet composed of saturated fatty acids (SFAs) has significant detrimental effects on brain homeostasis, and deleterious effects of SFAs on various cells have been well documented. However, the effects of SFAs on neural stem Cells (NSCs) function have not been fully explored. The aim of this study was to determine whether palmitic acid (PA) affected the proliferation and differentiation of murine‐derived NSCs. The results showed that PA dose dependently suppressed viability of NSCs and was cytotoxic at high concentrations. The toxic levels of PA inhibited the proliferation of NSCs as shown by reduced bromodeoxyuridine labeling of NSCs, which is correlated with reactive oxygen species generation. Pretreatment of the cells with the antioxidant N‐acetyl‐L‐cysteine inhibitor significantly attenuated the effects of PA on the proliferation of NSCs. Furthermore, nontoxic levels of PA promoted astrocytogenesis in the differentiated NSCs, associated with Stat3 activation and altered expression of serial of basic helix–loop–helix transcription factor genes. Altogether, our data have demonstrated that PA has a significant impact on proliferation and differentiation of NSCs in vitro and may be useful for elucidating the role of SFAs in regulating NSCs fate in physiological and pathological settings. © 2014 Wiley Periodicals, Inc.