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Upregulation of SYF2 is associated with neuronal apoptosis caused by reactive astrogliosis to neuroinflammation
Author(s) -
Xu Wei,
Cao Maohong,
Zheng Heyi,
Tan Xiang,
Li Lei,
Cui Gang,
Xu Jian,
Cao Jianhua,
Ke Kaifu,
Wu Qiyun
Publication year - 2014
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23312
Subject(s) - astrogliosis , neuroinflammation , downregulation and upregulation , microbiology and biotechnology , astrocyte , apoptosis , signal transduction , neuroscience , lipopolysaccharide , biology , chemistry , inflammation , immunology , central nervous system , biochemistry , gene
SYF2, known as CCNDBP1‐interactor or p29 , is likely involved in pre‐mRNA splicing and cell cycle progression. The present study was designed to elucidate dynamic changes in SYF2 expression and distribution in the cerebral cortex in a lipopolysaccharide (LPS)‐induced neuroinflammation rat model. It was found that SYF2 expression was induced strongly in active astrocytes after LPS injection. In vitro studies showed that the upregulation of SYF2 might be involved in the activation of C6 cells after LPS challenge and the neuronal apoptosis after conditioned media challenge. In addition, with silencing of SYF2 in C6 and PC12 cells by siRNA, the results indicated that SYF2 was required for astrocyte activation and neuronal apoptosis induced by LPS. Our findings on the cellular signaling pathway may provide a new therapeutic strategy against neuroinflammation in the CNS. © 2013 Wiley Periodicals, Inc.