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Feasibility of direct mapping of cerebral fluorodeoxy‐D‐glucose metabolism in situ at subcellular resolution using soft X‐ray fluorescence
Author(s) -
PoitryYamate Carole,
Giacelli Alessandra,
Kaulich Burkhard,
Kourousias George,
Magill Arthur W.,
Lepore Mario,
Gajdosik Vincent,
Gruetter Rolf
Publication year - 2013
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23171
Subject(s) - positron emission tomography , fluorescence , image resolution , in vivo , carbohydrate metabolism , resolution (logic) , glucose uptake , metabolism , biophysics , chemistry , fluorescence lifetime imaging microscopy , biology , nuclear magnetic resonance , nuclear medicine , biochemistry , neuroscience , medicine , physics , optics , endocrinology , microbiology and biotechnology , artificial intelligence , computer science , insulin
Glucose metabolism is difficult to image with cellular resolution in mammalian brain tissue, particularly with 18 fluorodeoxy‐D‐glucose (FDG) positron emission tomography (PET). To this end, we explored the potential of synchrotron‐based low‐energy X‐ray fluorescence (LEXRF) to image the stable isotope of fluorine (F) in phosphorylated FDG (DG‐6P) at 1 μm 2 spatial resolution in 3‐μm‐thick brain slices. The excitation‐dependent fluorescence F signal at 676 eV varied linearly with FDG concentration between 0.5 and 10 mM, whereas the endogenous background F signal was undetectable in brain. To validate LEXRF mapping of fluorine, FDG was administered in vitro and in vivo, and the fluorine LEXRF signal from intracellular trapped FDG‐6P over selected brain areas rich in radial glia was spectrally quantitated at 1 μm 2 resolution. The subsequent generation of spatial LEXRF maps of F reproduced the expected localization and gradients of glucose metabolism in retinal Müller glia. In addition, FDG uptake was localized to periventricular hypothalamic tanycytes, whose morphological features were imaged simultaneously by X‐ray absorption. We conclude that the high specificity of photon emission from F and its spatial mapping at ≤1 μm resolution demonstrates the ability to identify glucose uptake at subcellular resolution and holds remarkable potential for imaging glucose metabolism in biological tissue. © 2012 Wiley Periodicals, Inc.