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Dynamic change of numbl expression after sciatic nerve crush and its role in Schwann cell differentiation
Author(s) -
Zhu Lin,
Yan Yaohua,
Ke Kaifu,
Wu Xinming,
Gao Yilu,
Shen Aiguo,
Li Jing,
Kang Lihua,
Zhang Guowei,
Wu Qiyun,
Yang Huiguang
Publication year - 2012
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23039
Subject(s) - sciatic nerve , schwann cell , sciatic nerve injury , peripheral nervous system , microbiology and biotechnology , cellular differentiation , biology , nerve injury , downregulation and upregulation , wallerian degeneration , neuroscience , nervous system , peripheral nerve injury , anatomy , central nervous system , biochemistry , gene
Numbl, as a conserved homolog of Drosophila Numb, has been implicated in early development of the nervous system, but its expression and roles in nervous system lesion and repair remained unknown. Here, we performed an acute sciatic nerve injury model in adult rats and studied the dynamic changes of Numbl expression in the sciatic nerve. Temporally, Numbl expression was sharply decreased after sciatic nerve crush and reached a valley at day 7. Spatially, Numbl was widely expressed in the normal sciatic nerve, including axons and Schwann cells, whereas, after injury, Numbl expression was decreased predominantly in Schwann cells. In vitro, we induced Schwann cell differentiation with cAMP and found that Numbl expression was decreased in the differentiated process. Depletion of Numbl could promote Schwann cell differentiation. In addition, we demonstrated that in vitro myelination was suppressed by overexpression of Numbl in Schwann cells. Collectively, we hypothesized peripheral nerve injury induced a downregulation of Numbl in the sciatic nerve, which was associated with Schwann cell differentiation. © 2012 Wiley Periodicals, Inc.