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Neuropeptide Y and peptide YY have distinct roles in adult mouse olfactory neurogenesis
Author(s) -
Doyle Kharen L.,
Hort Yvonne J.,
Herzog Herbert,
Shine John
Publication year - 2012
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.23008
Subject(s) - neuropeptide y receptor , peptide yy , neurogenesis , biology , endocrinology , medicine , olfactory ensheathing glia , doublecortin , olfaction , olfactory system , neuron , neuropeptide , neuroscience , receptor , olfactory bulb , central nervous system , dentate gyrus
Abstract Neuropeptide Y (NPY) and peptide YY (PYY) are differentially expressed throughout the olfactory neuroepithelium (ON), with NPY expression present in sustentacular cells, olfactory ensheathing cells, and olfactory receptor neurons and PYY expressed only in sustentacular cells. Examination of the anatomical morphology of the ON in NPY knockout (NPY −/− ) and PYY knockout (PYY −/− ) mice shows that there are significantly more neurons in PYY −/− mice and significantly fewer neurons in NPY −/− mice. Interestingly, the mature neurons of NPY −/− mice were undergoing apoptosis. The transcription factor Mash1, which is critical in the production of olfactory precursors, is also differentially expressed in NPY −/− and PYY −/− ON. It is upregulated in the neurons of NPY −/− mice and unchanged in PYY −/− mice. Furthermore, significantly fewer olfactory neurospheres are present in cultures prepared from PYY −/− mice in the first 2 weeks compared with NPY −/− and wild‐type mice. Together these results suggest that, during olfactory neurogenesis, NPY acts as a trophic factor for the maturation and survival of olfactory receptor neurons, whereas PYY has an important role in the regulation of olfactory neuron differentiation. © 2012 Wiley Priodicals, Inc.