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Intersectin1‐s is involved in migration and invasion of human glioma cells
Author(s) -
Ma Yongjie,
Wang Bingbing,
Li Wenliang,
Liu Xiaoli,
Wang Jing,
Ding Ting,
Zhang Jiao,
Ying Guoguang,
Fu Li,
Gu Feng
Publication year - 2011
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22616
Subject(s) - cell migration , endocytosis , glioma , exocytosis , microbiology and biotechnology , cofilin , biology , integrin , actin cytoskeleton , cancer research , chemistry , cytoskeleton , cell , genetics , secretion , biochemistry
Malignant gliomas have a tendency to invade diffusely into surrounding healthy brain tissues, thereby precluding their successful surgical removal. Intersectin1 (ITSN1) as a molecular linker in the central nervous system is well known as an important regulator of endocytosis and exocytosis. ITSN1 has two isoforms: ITSN1‐l and ITSN1‐s. In this study, we show that siRNA‐mediated down regulation of ITSN1‐s inhibited migration and invasion of glioma cells. In addition, we demonstrate the possible mechanisms by which ITSN1‐s functions in migration and invasion. Several key proteins, including cofilin, LIMK, PAK, FAK, integrin β1, and MMP‐9, which are critical for cells migration and invasion, were probably involved in ITSN1‐s signaling pathways. These results suggest that ITSN1‐s contributes to glioma cells migration and invasion by regulating the formation of cytoskeleton, influencing adhesion and increasing expression of MMP‐9. Our results indicate that ITSN1‐s is a critical factor in glioms invasion and identify that ITSN1‐s is a new potentially antiinvasion target for therapeutic intervention in gliomas. © 2011 Wiley‐Liss, Inc.