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Hypoxic‐ischemic brain injury exacerbates neuronal apoptosis and precipitates spontaneous seizures in glucose transporter isoform 3 heterozygous null mice
Author(s) -
Fung Camille,
Evans Edward,
Shin Don,
Shin BoChul,
Zhao Yuanzi,
Sankar Raman,
Chaudhuri Gautam,
Devaskar Sherin U.
Publication year - 2010
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22487
Subject(s) - glut3 , glut1 , glucose transporter , endocrinology , medicine , ischemia , apoptosis , brain ischemia , biology , chemistry , anesthesia , biochemistry , insulin
We examined the effects of 45‐min hypoxia (FiO 2 0.08; Hx) vs. normoxia (FiO 2 0.21; Nx) on the ipsilateral (Ipsi) and contralateral (Ctrl) sides of the brain in neuronal glucose transporter isoform 3 (Glut3) heterozygous null mice (glut3 +/− ) and their wild‐type littermates (WT), undergoing unilateral carotid artery ligation. Glut3 +/− mice, under Nx, demonstrated a compensatory increase in blood–brain barrier/glial Glut1 protein concentration and a concomitant increase in neuronal nitric oxide synthase (nNOS) enzyme activity and Bax protein, with a decrease in procaspase 3 protein ( P < 0.05 each). After Hx, reoxygenation in FiO 2 of 0.21 led to no comparable adaptive up‐regulation of the ipsilateral brain Glut3 or Glut1 protein at 4 hr and Glut1 at 24 hr in glut3 +/− vs. WT. These brain Glut changes in glut3 +/− but not WT mice were associated with an increase in proapoptotic Bax protein and caspase‐3 enzyme activity ( P < 0.01 each) and a decline in the antiapoptotic Bcl‐2 and procaspase‐3 proteins ( P < 0.05 each). Glut3 +/− mice after Hx demonstrated TUNEL‐positive neurons with nuclear pyknosis in most ipsilateral (hypoxic‐ischemia) brain regions. A subset (∼55%) of glut3 +/− mice developed spontaneous seizures after hypoxic‐ischemia, confirmed by electroencephalography, but the WT mice remained seizure‐free. Pentylenetetrazole testing demonstrated an increased occurrence of longer lasting clinical seizures at a lower threshold in glut3 +/− vs. WT mice, with no detectable differences in monamine neurotransmitters. We conclude that hypoxic‐ischemic brain injury in glut3 +/− mice exacerbates cellular apoptosis and necrosis and precipitates spontaneous seizures. © 2010 Wiley‐Liss, Inc.