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Reduction of hippocampal N‐acetyl aspartate level in aged APP Swe /PS1 dE9 transgenic mice is associated with degeneration of CA3 pyramidal neurons
Author(s) -
Xu Wei,
Zhan Yanqiang,
Huang Wei,
Wang Xuxia,
Zhang Sumin,
Lei Hao
Publication year - 2010
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22479
Subject(s) - nissl body , hippocampal formation , hippocampus , genetically modified mouse , degeneration (medical) , intracellular , in vivo , pathology , creatine , staining , biology , chemistry , endocrinology , transgene , medicine , biochemistry , microbiology and biotechnology , gene
Age‐related metabolic changes in the hippocampus of APP Swe /PS1 dE9 mice were measured with long echo‐time in vivo 1 H‐magnetic resonance spectroscopy ( 1 H‐MRS). Thioflavine S staining and Nissl staining were used to characterize deposition of Aβ aggregates and neuronal degeneration in the transgenic animals, respectively. The results showed that the APP Swe /PS1 dE9 mice had significantly decreased hippocampal N‐acetyl aspartate (NAA)/total creatine (tCr) level at 16 months of age, which was associated with degeneration of and intracellular deposition of thioflavine S‐positive materials in hippocampal CA3 pyramidal neurons. The results of this study provide direct evidence showing association among Aβ pathology (intracellular deposition of thioflavine S‐positive materials), neuronal degeneration, and metabolic changes observable with in vivo 1 H‐MRS in the hippocampus of APP Swe /PS1 dE9 mice. © 2010 Wiley‐Liss, Inc.