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Stromal cell‐derived factor‐1 promotes migration of cells from the upper rhombic lip in cerebellar development
Author(s) -
Yu Tao,
Huang Hai,
Li HuiFang
Publication year - 2010
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22454
Subject(s) - granule cell , microbiology and biotechnology , stromal cell , chemotaxis , progenitor cell , cell migration , granule (geology) , cell , motility , biology , stem cell , chemistry , neuroscience , central nervous system , cancer research , genetics , receptor , paleontology , dentate gyrus
During cerebellar development, the chemokine stromal cell‐derived factor‐1α (SDF‐1α) has been shown to play an important role in recruiting cells from the upper rhombic lip (URL) and external granule cell layer (EGL). However, its function in cerebellar development is still poorly understood. Our results have demonstrated that SDF‐1 is necessary for EGL development, and URL cells stream to the SDF‐1 source in vitro. Results of embryonic URL explant assays and transwell assays indicated that SDF‐1 induces neural cell migration from the URL region in chemotactic and chemokinetic responses. The time‐lapse results showed that the migration speed of granule cell progenitors out of the URL was accelerated by the addition of recombinant SDF‐1α. Collectively, our study shows that SDF‐1 increases the motility of URL cells in the absence of a gradient and promotes the migration of granule cell progenitors during cerebellar development. © 2010 Wiley‐Liss, Inc.