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Intraarterially delivered human umbilical cord blood‐derived mesenchymal stem cells in canine cerebral ischemia
Author(s) -
Chung DaiJung,
Choi ChiBong,
Lee SungHo,
Kang EunHee,
Lee JaeHoon,
Hwang SooHan,
Han Hoon,
Lee JongHwan,
Choe BoYoung,
Lee SooYeol,
Kim HwiYool
Publication year - 2009
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22162
Subject(s) - medicine , transplantation , mesenchymal stem cell , basilar artery , ischemia , umbilical cord , von willebrand factor , cerebral infarction , neurotrophic factors , vascular endothelial growth factor , infarction , neuroprotection , anesthesia , pathology , platelet , anatomy , myocardial infarction , receptor , vegf receptors
The present study examined the effects of human umbilical cord blood‐derived mesenchymal stem cells (HUCB‐derived MSCs) delivered through the basilar artery in a canine thromboembolic brain ischemia model. Cerebral ischemia was induced through occlusion of the middle cerebral artery by injecting thrombus emboli into 10 beagles. In the HUCBC group (n = 5), 1 × 10 6 HUCB‐derived MSCs were transplanted through the basilar artery 1 day after ischemic induction using an endovascular interventional approach. In the control group (n = 5), phosphate‐buffered saline (PBS) was injected in the same manner in as the HUCBC group. Upon neurobehavioral examination, earlier recovery was observed in the HUCBC group. The HUCBC group showed a decrease in the infarction volume at 1 week after cerebral ischemic induction, whereas the control group showed an increase in the infarction volume at 1 week, by magnetic resonance image analysis. Transplanted cells had differentiated into neurons and astrocytes and were observed in and around endothelial cells that were positive for von Willebrand factor (vWF). HUCB‐derived MSCs expressed neuroprotective factors, such as brain‐derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), at 4 weeks after the transplantation. The transplanted cells demonstrated their efficacy by reducing the infarction lesion volume and through earlier recovery from the neurological deficit. These results suggest that intraarterial transplantation of HUCB‐derived MSCs could be useful in clinical treatment of cerebral ischemia. © 2009 Wiley‐Liss, Inc.