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Interleukin‐6 increases intracellular Ca 2+ concentration and induces catecholamine secretion in rat carotid body glomus cells
Author(s) -
Fan Juan,
Zhang Bo,
Shu HaiFeng,
Zhang XiaoYu,
Wang Xi,
Kuang Fang,
Liu Ling,
Peng ZhengWu,
Wu Rui,
Zhou Zhuan,
Wang BaiRen
Publication year - 2009
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22107
Subject(s) - glomus cell , carotid body , proinflammatory cytokine , receptor , catecholamine , extracellular , endocrinology , biology , medicine , stimulation , calcium in biology , secretion , chemoreceptor , intracellular , chemistry , microbiology and biotechnology , inflammation , immunology
Although abundant evidence indicates mutual regulation between the immune and the central nervous systems, how the immune signals are transmitted to the brain is still an unresolved question. In a previous study we found strong expression of proinflammatory cytokine receptors, including interleukin (IL)‐1 receptor I and IL‐6 receptor α in the rat carotid body (CB), a well‐known arterial chemoreceptor that senses a variety of chemostimuli in the arterial blood. We demonstrated that IL‐1 stimulation increases intracellular calcium ([Ca 2+ ] i ) in CB glomus cells, releases ATP, and increases the discharge rate in carotid sinus nerve. To explore the effect of IL‐6 on CB, here we examine the effect of IL‐6 on [Ca 2+ ] i and catecholamine (CA) secretion in rat CB glomus cells. Calcium imaging showed that extracellular application of IL‐6 induced a rise in [Ca 2+ ] i in cultured glomus cells. Amperometry showed that local application of IL‐6 evoked CA release from glomus cells. Furthermore, the CA secretory response to IL‐6 was blocked by 200 μM Cd 2+ , a well‐known Ca 2+ channel blocker. Our experiments provide further evidence for the responsiveness of the CB to proinflammatory cytokines and indicate that the CB might play a role in inflammation sensing and transmission of such information to the brain. © 2009 Wiley‐Liss, Inc.