Premium
γ‐Aminobutyric acid‐mediated regulation of the activity‐dependent olfactory bulb dopaminergic phenotype
Author(s) -
Akiba Yosuke,
Sasaki Hayato,
Huerta Patricio T.,
Estevez Alvaro G.,
Baker Harriet,
Cave John W.
Publication year - 2009
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.22055
Subject(s) - olfactory bulb , gabaergic , dopaminergic , biology , neurogenesis , microbiology and biotechnology , neuroscience , interneuron , inhibitory postsynaptic potential , dopamine , central nervous system
γ‐Aminobutyric acid (GABA) regulates the proliferation and migration of olfactory bulb (OB) interneuron progenitors derived from the subventricular zone (SVZ), but the role of GABA in the differentiation of these progenitors has been largely unexplored. This study examines the role of GABA in the differentiation of OB dopaminergic interneurons using neonatal forebrain organotypic slice cultures prepared from transgenic mice expressing green fluorescent protein (GFP) under the control of the tyrosine hydroxylase ( Th ) gene promoter ( Th GFP). KCl‐mediated depolarization of the slices induced Th GFP expression. The addition of GABA to the depolarized slices further increased GFP fluorescence by inducing Th GFP expression in an additional set of periglomerular cells. These findings show that GABA promoted differentiation of SVZ‐derived OB dopaminergic interneurons and suggest that GABA indirectly regulated Th expression and OB dopaminergic neuron differentiation through an acceleration of the maturation rate for the dopaminergic progenitors. Additional studies revealed that the effect of GABA on Th GFP expression required activation of L‐ and P/Q‐type Ca 2+ channels as well as GABA A and GABA B receptors. These voltage‐gated Ca 2+ channels and GABA receptors have previously been shown to be required for the coexpressed GABAergic phenotype in the OB interneurons. Together, these findings suggest that Th expression and the differentiation of OB dopaminergic interneurons are coupled to the coexpressed GABAergic phenotype and demonstrate a novel role for GABA in neurogenesis. © 2009 Wiley‐Liss, Inc.