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Traumatic brain injury alters expression of hippocampal microRNAs: Potential regulators of multiple pathophysiological processes
Author(s) -
Redell John B.,
Liu Yin,
Dash Pramod K.
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21945
Subject(s) - microrna , biology , neurogenesis , microarray , traumatic brain injury , signal transduction , pathophysiology , microarray analysis techniques , hippocampal formation , gene expression , neuroscience , microbiology and biotechnology , bioinformatics , gene , medicine , genetics , endocrinology , psychiatry
Multiple cellular, molecular, and biochemical changes contribute to outcome after traumatic brain injury (TBI). MicroRNAs (miRNAs) are known to influence many important cellular processes, including proliferation, apoptosis, neurogenesis, angiogenesis, and morphogenesis, all processes that are involved in TBI pathophysiology. However, it has not yet been determined whether miRNA expression is altered after TBI. In the present study, we used a microarray platform to examine changes in the hippocampal expression levels of 444 verified rodent miRNAs at 3 and 24 hr after controlled cortical impact injury. Our analysis found 50 miRNAs exhibited decreased expression levels and 35 miRNAs exhibited increased expression levels in the hippocampus after injury. We extended the microarray findings using quantitative polymerase chain reaction analysis for a subset of the miRNAs with altered expression levels (miR‐107, ‐130a, ‐223, ‐292‐5p, ‐433‐3p, ‐451, ‐541, and ‐711). Bioinformatic analysis of the predicted targets for this panel of miRNAs revealed an overrepresentation of proteins involved in several biological processes and functions known to be initiated after injury, including signal transduction, transcriptional regulation, proliferation, and differentiation. Our results indicate that multiple protein targets and biological processes involved in TBI pathophysiology may be regulated by miRNAs. © 2008 Wiley‐Liss, Inc.