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Increased oxidation of certain glycolysis and energy metabolism enzymes in the frontal cortex in Lewy body diseases
Author(s) -
Gómez Anna,
Ferrer Isidre
Publication year - 2009
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21904
Subject(s) - aldolase a , enolase , glyceraldehyde 3 phosphate dehydrogenase , glycolysis , glyceraldehyde , frontal cortex , fructose bisphosphate aldolase , lewy body , frontal lobe , dementia with lewy bodies , carbohydrate metabolism , cortex (anatomy) , medicine , enzyme , endocrinology , biochemistry , chemistry , biology , parkinson's disease , dehydrogenase , immunohistochemistry , dementia , neuroscience , disease
Lipoxidative damage of aldolase A, enolase 1, and glyceraldehyde dehydrogenase (GAPDH) was found in the frontal cortex in a percentage of aged controls by bidimensional gel electrophoresis, Western blot test, in‐gel digestion, and mass spectrometry. Aldolase A and enolase 1 were altered in 12 of 19 cases, whereas oxidation of GAPDH was found in 6 of 19 controls. The three enzymes were oxidized in the frontal cortex in the majority of cases of incidental Parkinson's disease (iPD), PD, and dementia with Lewy bodies (DLB). Differences were statistically significant (χ 2 test) for GAPDH in PD and DLB. Densitometric studies have shown that the ratio of oxidized protein per spot is higher in iPD, PD, and DLB compared with controls. These findings show oxidation of three enzymes linked with glycolysis and energy metabolism in the adult human brain as well as increased oxidation of aldolase A, enolase 1, and GAPDH in the frontal cortex in Lewy body diseases. Modifications of these enzymes may result in decreased activity and may partly account for impaired metabolism and function of the frontal lobe in PD. © 2008 Wiley‐Liss, Inc.