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Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo
Author(s) -
Masuda Tomoyuki,
Sakuma Chie,
Kobayashi Kenta,
Kikuchi Kazufumi,
Soda Emi,
Shiga Takashi,
Kobayashi Kazuto,
Yaginuma Hiroyuki
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21868
Subject(s) - spinal cord , microbiology and biotechnology , dorsal root ganglion , laminin , receptor , biology , neuroscience , dorsum , embryo , peptide , central nervous system , cord , anatomy , medicine , biochemistry , surgery , extracellular matrix
During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We previously reported the involvement of dorsal spinal cord‐derived chemoattraction in this projection (Masuda et al. [ 2007] Neuroreport 18:1645–1649). However, the molecular nature of this attraction is not clear. Here we show that laminin‐1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67‐kDa receptor (67LR) is present on DRG cells. This evidence suggests that laminin‐1‐67LR signaling may be involved in DRG axonal guidance. By employing culture assays, we show that laminin‐1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promotes the DRG axonal response to dorsal spinal cord‐derived chemoattraction. By using a function‐blocking antibody against 67LR, we show that the anti‐67LR antibody blocks the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti‐67LR antibody inhibits the DRG axonal growth toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord‐derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons. © 2008 Wiley‐Liss, Inc.